Data/hora: 18/05/2024 22:44:48

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 21/03/1998
Data da última atualização: | 21/03/1998
Autoria: | PIFFER, I. A.; ROSS, R. F.
Afiliação: | CNPSA.
Título: | Immunofluorescente technique for detection of mycoplasma hyopneumoniae in swine lungs.
Ano de publicação: | 1985
Fonte/Imprenta: | Pesquisa Agropecuaria Brasileira, Brasilia, v.20, n.8, p.877-882, ago. 1985.
Idioma: | Inglês
Conteúdo: | Direct and indirect immunofluorescence techniques and a culture method were compared for detection of Mycoplasma hyopneumoniae in lungs of experimentally infected swine. Pneumonic lungs were obtained from SPF pigs that had been exposed by contact 6 to 7 weeks previously to other SPF pigs which had been inoculated with the organism. A good association was found between isolation of M. hyopneumoniae, positive immunofluorescence test results and presence of macroscopic or microscopic lesions typical of my coplasmal pneumonia of swine. Direct and indirect immunofluorescence tests were equally efficient for detection of M. hyopneumoniae in sections of lung. An azo dye counterstain reduced  nonspecific fluorescence and provided a good color contrast without reducing sensitivity of the indirect immunofluorescence test.
Palavras-Chave: | Diagnosis; Enzootic pneumonia; Mycoplasmal pneumonia; Pneumonia micoplasmica.
Thesagro: | Diagnostico; Pneumonia Enzoótica.
Categoria do assunto: | --
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (19758-1) - UMT - AP - -- - 630.72081 - P474 - 630.7208 - DIS